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Image Search Results
Journal: Scientific reports
Article Title: Noggin promotes osteogenesis in human adipose-derived mesenchymal stem cells via FGFR2/Src/Akt and ERK signaling pathway.
doi: 10.1038/s41598-024-56858-w
Figure Lengend Snippet: Figure 1. Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of (a) normal human bone marrow stromal cells (BMSCs), (b) normal human dental pulp stem cells (DPSCs) and (c) human immortalized adipose-derived stem cell line (ASC52telo). Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid (Asc) and dexamethasone (Dex). (d) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). (a–d) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in (e) normal human ASCs and (f) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. (a–f) Average values ± SD are plotted. One-way ANOVA tests, *p < 0.05, **p < 0.001, ***p < 0.0001, ns—not significant, relative to respective control or between marked groups.
Article Snippet:
Techniques: Activity Assay, Expressing, Derivative Assay, Recombinant, Control, Quantitative Proteomics, Cell Culture