human recombinant noggin Search Results


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Recombinant Human Noggin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Noggin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene recombinant human noggin
Figure 1. Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of (a) normal human bone marrow stromal cells (BMSCs), (b) normal human dental pulp stem cells (DPSCs) and (c) human immortalized adipose-derived stem cell line (ASC52telo). Cells were treated with either 100 ng/ml <t>recombinant</t> human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid (Asc) and dexamethasone (Dex). (d) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). (a–d) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in (e) normal human ASCs and (f) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. (a–f) Average values ± SD are plotted. One-way ANOVA tests, *p < 0.05, **p < 0.001, ***p < 0.0001, ns—not significant, relative to respective control or between marked groups.
Recombinant Human Noggin, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novoprotein wnt 3a novoprotein cat
Figure 1. Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of (a) normal human bone marrow stromal cells (BMSCs), (b) normal human dental pulp stem cells (DPSCs) and (c) human immortalized adipose-derived stem cell line (ASC52telo). Cells were treated with either 100 ng/ml <t>recombinant</t> human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid (Asc) and dexamethasone (Dex). (d) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). (a–d) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in (e) normal human ASCs and (f) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. (a–f) Average values ± SD are plotted. One-way ANOVA tests, *p < 0.05, **p < 0.001, ***p < 0.0001, ns—not significant, relative to respective control or between marked groups.
Wnt 3a Novoprotein Cat, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Regeneron inc recombinant human noggin
Figure 1. Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of (a) normal human bone marrow stromal cells (BMSCs), (b) normal human dental pulp stem cells (DPSCs) and (c) human immortalized adipose-derived stem cell line (ASC52telo). Cells were treated with either 100 ng/ml <t>recombinant</t> human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid (Asc) and dexamethasone (Dex). (d) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). (a–d) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in (e) normal human ASCs and (f) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. (a–f) Average values ± SD are plotted. One-way ANOVA tests, *p < 0.05, **p < 0.001, ***p < 0.0001, ns—not significant, relative to respective control or between marked groups.
Recombinant Human Noggin, supplied by Regeneron inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of (a) normal human bone marrow stromal cells (BMSCs), (b) normal human dental pulp stem cells (DPSCs) and (c) human immortalized adipose-derived stem cell line (ASC52telo). Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid (Asc) and dexamethasone (Dex). (d) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). (a–d) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in (e) normal human ASCs and (f) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. (a–f) Average values ± SD are plotted. One-way ANOVA tests, *p < 0.05, **p < 0.001, ***p < 0.0001, ns—not significant, relative to respective control or between marked groups.

Journal: Scientific reports

Article Title: Noggin promotes osteogenesis in human adipose-derived mesenchymal stem cells via FGFR2/Src/Akt and ERK signaling pathway.

doi: 10.1038/s41598-024-56858-w

Figure Lengend Snippet: Figure 1. Noggin protein increases alkaline phosphatase (ALP) activity and early osteogenic genes expression in adult human mesenchymal stem cells from different tissues. Alkaline phosphatase (ALP) activity after 7-d culture of (a) normal human bone marrow stromal cells (BMSCs), (b) normal human dental pulp stem cells (DPSCs) and (c) human immortalized adipose-derived stem cell line (ASC52telo). Cells were treated with either 100 ng/ml recombinant human Noggin (NOG) or 100 ng/ml recombinant human bone morphogenetic protein 2 (BMP-2), or both (BMP-2 + NOG), in osteogenic medium containing ascorbic acid (Asc) and dexamethasone (Dex). (d) ALP activity after 7-d culture of ASC52telo cells in osteogenic medium with different Noggin doses (100–400 ng/ml). (a–d) Control represents cells maintained in standard growth medium. Relative mRNA levels (qPCR) of selected osteoblastic markers in (e) normal human ASCs and (f) normal human BMSCs continuously treated with Noggin (NOG) or BMP-2 for 7 days in osteogenic medium. Relative quantification to control cells cultured in osteogenic medium. (a–f) Average values ± SD are plotted. One-way ANOVA tests, *p < 0.05, **p < 0.001, ***p < 0.0001, ns—not significant, relative to respective control or between marked groups.

Article Snippet: Recombinant human Noggin (rhNoggin, Origene) or recombinant human BMP-2 (rhBMP-2, ThermoFisher Scientific) were added from day 1 cultures at concentrations of 100 ng/ml, unless indicated otherwise.

Techniques: Activity Assay, Expressing, Derivative Assay, Recombinant, Control, Quantitative Proteomics, Cell Culture